General description
GABA(A) receptor subunit gamma-2 is a protein belonging to a subclass of GABA receptors known as GABA (A). GABA (A) receptors are pentameric chloride channels composed of subunits belonging to different homology classes, i.e. α, β, γ, δ, θ, ε, π, and act as receptors for GABA, a crucial inhibitory neurotransmitter localized within the brain. GABA (A) gamma 2 subunit consists of 5 cysteine residues and carries the binding site for benzodiazepine. The subunit functions to promote receptor maintenance and clustering at the synapse, interacts with GABA receptor associated proteins such as GODZ and GABAWRAP, affects GABA (A) receptor conductance and kinetics, and is a contributing component to receptors participating in both phasic and tonic inhibition. Several mutations in the GABA(A) receptor subunit gamma-2 expression have been linked to epileptic syndromes that result in fibrile seizures.
~54 kDa observed
Immunogen
Linear peptide corresponding to rat GABA(A) receptor subunit gamma-2.
Application
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected GABA(A) receptor subunit gamma-2 in rat hippocampal neurons. Image courtesy from the laboratory of Dr. Jaideep Kapur, University of Virginia.
Detect GABA(A) receptor subunit gamma-2 using this Anti-GABA(A) receptor subunit gamma-2 Antibody, clone 10F10-C1-B8 validated for use in Western Blotting, ICC.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blot in PC-12 cell lysate.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected GABA(A) receptor subunit gamma-2 in 10 µg of PC-12 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 10F10-C1-B8, monoclonal |
| species reactivity | rat |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NP_899156 |
| UniProt accession no. | P18508 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | rat ... Gabrg2(29709) |
