General description
H2AX is involved in DNA repair and the maintenance of genomic stability. It is been implicated both in homologous recombination and nonhomologous end joining DNA repair pathways. H2AX is thought to have a critical function in the recruitment of DNA repair factors and DNA damage-signaling proteins. H2A.X is ubiquitinated by RING1 and RNF2/RING2 complex providing a specific mark for transcriptional repression. In addition this protein can be ubiquitinated by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168 as result of double-stranded DNA breaks to recruit repair proteins a damaged site. H2AX is phosphorylated soon after the appearance of double-stranded breaks. Hyperphoshorylation of H2AX may be linked to chromatin fragmentation prior to apoptosis.
~ 23 kDa
Immunogen
Epitope: Lys119
Linear peptide containing ubiquitylated Lys119 of human Histone H2A.X.
Application
Anti-Ubiquityl Histone H2A.X (Lys119) Antibody is a rabbit polyclonal antibody for detection of Ubiquityl Histone H2A.X (Lys119) also known as H2AXK119Ub, Histone H2A.X Ubiquityl K119) & has been validated in WB, IP, ICC.
Immunocytochemsitry Analysis: 1:500 dilution from a representative lot detected Histone H2A.X in RAW264.7 and NIH/3T3 cells.
Immunoprecipitation Analysis: 5 µl from a representative lot immunoprecipitated Histone H2A.X in HeLa acid extract.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Biochem/physiol Actions
This antibody recognizes Histone H2A.X when ubiquitylated at Lys119.
Physical form
Unpurified
Unpurified rabbit polyclonal containing serum with 0.05% sodium azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Analysis Note
Control
HeLa acid extract
Evaluated by Western Blot in HeLa acid extract.
Western Blot Analysis: 1:1,000 dilution of this antibody detected Histone H2A.X on 10 µg of HeLa acid extract.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | rat (based on 100% sequence homology), bovine (based on 100% sequence homology), canine (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| NCBI accession no. | NP_002096.1 |
| UniProt accession no. | P16104 |
| shipped in | wet ice |
| target post-translational modification | ubiquitination (Lys119) |
| Gene Information | bovine ... H2Ax(531733) dog ... H2Ax(489372) human ... H2AX(3014) mouse ... H2Ax(15270) rat ... H2Ax(500987) |
