General description
Gamma-1-Tubulin, also known as Gamma-tubulin complex component 1, GCP-1 or Tubulin gamma-1 chain, and encoded by the gene TUBG1/TUBG, is a form of tubulin protein and a component of microtubules. Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome and plays a major role in the organization of the microtubule cytoskeleton. Gamma-1-Tubulin is part of the pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, and it is also involved in centrosome duplication and spindle formation. Interestingly, gamma tubulin is often up regulated but improperly compartmentalized in many cancer cells including non-small cell lung cancer. Mutations in Gamma-1-Tubulin gene are associated with developmental cortical defects including microcephaly and malformations of cortical development (MCD). Gamma-1-Tubulin is localized to the cytoplasm and cytoskeleton components and expressed in a variety of cell types and tissues.
~56 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Immunogen
GST-tagged recombinant protein corresponding to human Gamma-1-Tubulin.
Application
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Gamma-1-Tubulin in NIH/3T3 cells.
Alexa Fluor™ is a registered trademark of Life Technologies
This Anti-Gamma-1-Tubulin antibody, clone 6H3.1 is validated for use in western blotting & ICC for the detection of Gamma-1-Tubulin.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected Gamma-1-Tubulin in 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 6H3.1, monoclonal |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_001061 |
| UniProt accession no. | P23258 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... TUBG1(7283) |
