General description
42kDa and 44kDa Observed
Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as Erk1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator of the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs), GPCR, and/or integrin stimulation. This activates the Ras-Raf-MEK-Erk pathway that results in the phosphorylation/activation of Erk1/2 (p44/p42) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 & Erk2, respectively).
Immunogen
Epitope: Phosphorylated Thr185/Phosphorylated Tyr187
Phosphorylated recombinant peptide corresponding to amino acids surrounding the pTEpY motif in the activation loop of human phospho-Erk1/2, in which the Thr and Tyr residues are phosphorylated
Application
Research Category
Signaling
Research Sub Category
MAP Kinases
This Milli-Mark Phospho-Erk1/2 antibody is validated for use in Flow & WB for the detection of the Milli-Mark Phospho-Erk1/2 protein.
Biochem/physiol Actions
Antibody recognizes Recognizes Erk 1 & 2 only when dually phosphorylated on its TxY activation motif.
Physical form
Protein A purified
Purified Rabbit monoclonal supernatant conjugated to phycoerythrin in buffer containing PBS with 0.05% sodium azide
Preparation Note
Stable for 6 months at 2-8°C from date of receipt. Protect from light.
Analysis Note
Control
Jurkat Cells
Evaluated by Flow Cytometry with Jurkat cells.
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | PE |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | AW39R, monoclonal |
| species reactivity | mouse, rat |
| species reactivity (predicted by homology) | human (based on 100% sequence homology) |
| manufacturer/tradename | Milli-Mark® |
| technique(s) | flow cytometry: suitable,western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NP_002736.3 |
| UniProt accession no. | P28482 |
| shipped in | wet ice |
| target post-translational modification | phosphorylation ((pThr202/pTyr204), (pThr185/pTyr187)) |
| Gene Information | human ... MAPK3(5595) |
