General description
The N418 antibody reacts with mouse CD11c, also known as integrin alpha X. This 150 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains which are differentially expressed. Integrin alpha X (CD11c) assembles with Integrin beta-2 (CD18) into a receptor complex known as CR4 which can bind and induce signaling through ICAMs and VCAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.The N418 antibody is widely used as a marker for CD11c expression on dendritic cells (DC), often in parallel with markers for CD11b, for identification of developmental stages and mature subsets of this cell type. CD11c is prominently expressed on tissue macrophages, and is also detected on some types of activated T cells and intestinal intraepithelial lymphocytes (IEL).
Immunogen
Immunogen information for this antibody is proprietary information.
Application
Anti-CD11c (Mouse), redFluor® 710, clone N418 Antibody detects level of CD11c and has been published and validated for use in Flow Cytometry.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Physical form
Purified Armenian Hamster monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Analysis Note
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.125 μg from a representative lot detected CD11c in one million C57Bl/6 splenocytes.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
redFluor is a registered trademark of Tonbo Biotechnologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | hamster (Armenian) |
| Quality Segment | 100 |
| conjugate | redFluor® 710 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | N418, monoclonal |
| species reactivity | mouse |
| packaging | antibody small pack of 25 μg |
| technique(s) | flow cytometry: suitable |
| isotype | IgG |
| UniProt accession no. | Q9QXH4 |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Itgax(16411) |
