General description
Centrosome-associated protein CEP250 (UniProt: Q9BV73; also known as 250 kDa centrosomal protein, Cep250, Centrosomal NEK2-associated protein 1, C-Nap1, Centrosomal protein 2) is encoded by the CEP250 (also known as CEP2, CNAP1) gene (Gene ID: 11190) inn human. Cep250 is a component of the core centrosome that is ubiquitously expressed. It can exist as a monomer and homodimer. It specifically associates with the proximal ends of both mother and daughter centrioles during interphase and in mitotic cells it dissociates from the mitotic spindle poles. At the end of cell division, it is shown to re-accumulate at centrosomes. Cep250 is differentially phosphorylated during cell cycle and phosphorylation status regulates its association/dissociation from centrosome. During M phase its C-terminal part is shown to be phosphorylated by NEK2. Two isoforms of Cep250 have been described that are produced by alternative splicing. Loss of Cep250-mediated centriole cohesion leads to an altered cell migration phenotype.
~280 kDa observed; 281.14 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Recombinant fragment corresponding to 455 amino acids from the C-terminal region of human Centrosome-associated protein CEP250.
Application
Anti-C-Nap1 (Cep250), clone 150-230-1, Cat. No. MABT1353, is a highly specific mouse monoclonal antibody that targets Centrosome-associated protein CEP250 and has been tested for use in Immunofluorescence and Western Blotting.
Immunofluorescence Analysis: A representative lot detected C-Nap1 (Cep250) in Immunofluorescence applications (Fava, L.L., et. al. (2017). Genes Dev. 31(1):34-45).
Western Blotting Analysis: A representative lot detected C-Nap1 (Cep250) in HeLa S3 cell lysates. (Courtesy of Prof. Erich A. Nigg, Ph.D., Biozentrum, University of Basel, Switzerland).
Biochem/physiol Actions
Clone 150-230-1 is a mouse monoclonal antibody that detects human Centrosome-associated protein CEP250 (Cep20). It targets an epitope within 455 amino acids from the C-terminal region.
Physical form
Format: Purified
Analysis Note
Isotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
Other Notes
Concentration: Please refer to lot specific datasheet.
| biological source | mouse |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 150-230-1, monoclonal |
| species reactivity | human |
| packaging | antibody small pack of 25 μg |
| technique(s) | immunofluorescence: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_001305148 |
| UniProt accession no. | Q9BV73 |
| target post-translational modification | unmodified |
| Gene Information | human ... CEP250(11190) |
