General description
S100A9, also known as Calgranulin-B, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, MRP-14, p14, or S100 calcium-binding protein A9, and encoded by the gene S100A9/CAGB/MRP14/S100-A9 is a calcium and zinc binding protein that plays a prominent role in the regulation of inflammatory processes and immune responses. S100-A9 can induce neutrophil chemotaxis and adhesion. S100-A9 one of the subunits of Calprotectin, which is a complex of S100-A8 and S100-A9 proteins and is also critical for immune responses. S100-A9 is a cytoplasmic and secreted protein predominantly expressed in myeloid cells as well as neutrophils and monocytes but not expressed in lymphocytes. S100-A9 is found in high concentrations at sites of inflammation or in the serum of patients with inflammatory diseases. S100-A9 is also a cancer marker as it is up regulated in many cancers including gastric, esophageal, colon, breast and many more.
~15 kDa observed
Immunogen
Recombinant protein corresponding to mouse S100A9.
Application
Activity Assay Analysis: A representative lot detected S100A9 and found that it increases neutrophil migration & induces the secretion of cytokines by monocytes (Cesaro, A., et al. (2012). PLOS ONE. 7(9):e45478 1-12).
Western Blotting Analysis: A representative lot detected S100A9 in mouse neutrophils and recombinant mS100A9 (Cesaro, A., et al. (2012). PLOS ONE. 7(9):e45478 1-12).
Anti-S100A9 Antibody, clone 2A5 (Azide Free) is an antibody against S100A9 for use in western blotting & activity assay.
Physical form
Format: Purified
Purified rat monoclonal IgG1 in buffer containing PBS without preservatives.
Analysis Note
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected S100A9 in 10 µg of Raji cell lysate.
| biological source | rat |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2A5, monoclonal |
| species reactivity | human, mouse |
| concentration | 1 mg/mL |
| technique(s) | activity assay: suitable,western blot: suitable |
| isotype | IgG1 |
| NCBI accession no. | NP_00126878 |
| UniProt accession no. | P31725 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... S100A9(6280) |
