General description
CUB domain-containing protein 1 (UniProt: Q9H5V8; also known as Membrane glycoprotein gp140, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Transmembrane and associated with Src kinases, CD318) is encoded by the CDCP1 (also known as TRASK) gene (Gene ID: 64866) in human. CUB domain-containing protein 1 is a single-pass membrane protein that is synthesized with a 29 amino acid signal peptide, which is subsequently cleaved to generate the mature form. A soluble form of CUB domain-containing protein 1 has also been reported that is produced by proteolytic cleavage at the cell surface. CUB domain-containing protein 1 is highly expressed in mitotic cells with low expression during interphase. It is detected at highest levels in skeletal muscle and colon. Lower expression is reported in kidney, small intestine, placenta, and lung. It is also reported in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells. CUB domain-containing protein 1 levels are up-regulated in a number of human tumor cell lines and in colorectal cancer, breast carcinoma and lung cancer. It is reported to be involved in cell adhesion and cell matrix association and may play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation.
92.93 kDa calculated.
Immunogen
His-tagged recombinant fragment corresponding to 638 amino acids from the extracellular domain of human CUB domain-containing protein 1.
Application
Anti-CDCP1 (CD318), clone 8A2, Cat. No. MABT859, is a mouse monoclonal antibody that detects CUB domain-containing protein 1 (CDCP1) and has been tested for use Flow Cytometry.
Flow Cytometry Analysis: A representative lot detected CDCP1 (CD318) in one million MDA and MDA KO cells (Courtesy of the Laboratory of Feng Lin, Ph.D., Cleveland Clinic, Lerner Research Institute, Cleveland OH USA).
Biochem/physiol Actions
Clone 8A2 detects CUB domain-containing protein 1 (CDCP1) in human cells. it targets an epitope within the extracellular domain.
Physical form
Format: Purified
Analysis Note
Evaluated by Flow Cytometry in DLD1 cells.
Flow Cytometry Analysis: 1 µg of this antibody detected CDCP1 (CD318) in one million DLD1 cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 8A2, monoclonal |
| species reactivity | human |
| packaging | antibody small pack of 25 μg |
| technique(s) | flow cytometry: suitable |
| isotype | IgG2bκ |
| web metadata keyword.default | Anti-CDCP1 antibody clone 8A2,CD318 antibody clone 8A2 for lab use,CD318 antibody clone 8A2,CDCP1 antibody 500µg for bioresearch,CDCP1 antibody clone 8A2 Sigma-Aldrich,CDCP1 antibody for immunological applications,CUB domain protein 1 antibody for molecular studies,CUB domain-containing protein 1 antibody 100µg,Sigma-Aldrich Anti-CDCP1 antibody for flow cytometry,anti-CD318 antibody for research,anti-CDCP1 antibody suitable for flow cytometry,anti-CDCP1 clone 8A2 for cell signaling research,flow cytometry reagent Anti-CDCP1 clone 8A2,high-purity Anti-CDCP1 antibody for clinical research |
| NCBI accession no. | NP_073753.3 |
| UniProt accession no. | Q9H5V8 |
| target post-translational modification | unmodified |
| Gene Information | human ... CDCP1(64866) |
