General description
Matrix metalloproteinases (MMPs) are a family of secreted and membrane-bound zinc endopeptidases. Collectively, these enzymes degrade the components of extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. MMPs play an important role in wound healing, apoptosis, bone elongation, embryo development, angiogenesis, cancer metastases, and tissue remodeling within many disease states.
Most MMP′s are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, MT1-MMP (MMP-14) is a member of the membrane-type subfamily. Each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. MT1-MMP is capable of mediating pericellular proteolysis of extracellular matrix components and is therefore thought to be an important molecular tool for cellular remodeling of the surrounding matrix. This protein also activates MMP2 protein, and this activity may be involved in tumor invasion.
~ 65 kDa
Immunogen
KLH conjugated synthetic peptide selected from the hinge region of human MT1-MMP.
Application
Detect MT1-MMP using this Anti-MT1-MMP Antibody, hinge region validated for use in WB, IH(P).
Biochem/physiol Actions
Predicted to cross react with mouse, (95% sequence homology) and monkey chimpanzee, canine and bovine (100% sequence homology). Reactivity with other species has not been tested.
The antibody recognizes human and rat MT1-MMP. It does not cross react with MMP-1, MMP-2, MMP-8, MMP-9, and MMP-13.
Analysis Note
Control
Rat lung lysate.
Evaluated on a representative lot by Western blot on rat lung lysate using Anti-MT1-MMP.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB815
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human, rat |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| UniProt accession no. | P50281 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... MMP14(4323) |
