General description
The protein named N-glycosylase/DNA lyase or alternatively, 8-oxoguanine (8-OxoG) DNA glycosylase or AP lyase, and encoded by the gene named OGG1/MMH/MUTM/OGH1, is a DNA repair enzyme that incises DNA at 8-oxoG residues and excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. OGG1 also has beta-lyase activity that nicks DNA 3′ to the lesion. OGG1 is localized to the nucleus and nucleoplasm; in UVA-irradiated cells it can be found in the nuclear speckles. There is also an isoform found in the mitochondrion. Interestingly, OGG1 appears to be play a critical role in repairing mtDNA damage; mtDNA damage is linked to mitochondrial dysfunction, increased oxidative stress, and insulin resistance in muscle cells, and studies using OGG1 knockouts or over expressing OGG1 demonstrate that OGG1 is important for proper mtDNA damage repair and that disruption of OGG1 can lead to increased mtROS (reactive oxygen species) production and subsequent regulation of downstream events leading to insulin resistance in skeletal muscle. OGG1, or rather defects in OGG1 function, are also associated with a number of carcinomas, particularly Renal Cell Carcinomas of various cell types.
~38 kDa observed
Immunogen
Recombinant protein corresponding to human OGG1.
Application
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-OGG1 Antibody is validated for use in Western Blotting for the detection of OGG1.
Western Blotting Analysis: A representative lot detected recombinant protein OGG1 (Bjoras, M., et al. (1997). EMBO. 16(20):6314–6322).
Western Blotting Analysis: A representative lot detected OGG1 in Melphalan-resistant cells (Sousa, M., et al. (2013). PLOS One. 8(2):e55493).
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in recombinant protein OGG1.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant protein OGG1 in 1 µg of cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| technique(s) | western blot: suitable |
| NCBI accession no. | NP_002533 |
| UniProt accession no. | O15527 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... OGG1(4968) |
