General description
Recombinant, human thioredoxin expressed in E. coli. A ubiquitous protein with two redox-active half-cysteine residues. Functions as a general disulfide reductant. Works efficiently on model compounds (e.g. Ellman’s reagent, Cat. No. 322123) and protein disulfides. Plays an essential role in cellular protection against oxidative stress and cell death. Inhibits apoptosis in Jurkat T cells and human PBL.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Reconstitute in physiological buffer containing 3 mM EDTA and 1-5 mM DTT.
Other Notes
Noda, N., et al. 1997. Nippon Rinsho 55, 461.
Wollman, E.E., et al. 1997. Biochem. Biophys. Res. Commun. 230, 602.
Masutani, H., et al. 1996. Immunol. Lett. 54, 67.
Saito, I., et al. 1996. Arthritis Rheumatism, 39, 773.
Saito, N., et al. 1995. J. Immunol. 154, 3194.
Buchanon, B.B., et al. 1994. Arch. Biochem. Biophys. 314, 257.
One unit (insulin reduction unit) is calculated by measuring the change in absorbance at 650 nm with 330 µM DTT as the reductant at 25°C, pH 6.5.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| recombinant | expressed in E. coli |
| Quality Level | 100 |
| description | Merck USA index - 14, 9358 |
| assay | ≥90% (SDS-PAGE) |
| form | lyophilized solid |
| specific activity | ≥1.7 units/mg protein |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze |
| solubility | soluble (Reconstitute in physiological buffer containing 3 mM EDTA and 1-5 mM DTT.) |
| shipped in | ambient |
| storage temp. | −20°C |
