General description
Tyrosine-protein kinase JAK1 (EC 2.7.10.2; UniProt P23458; also known as Janus kinase 1, JAK-1) is encoded by the JAK1 (also known as JAK1A, JAK1B) gene (Gene ID 3716) in human. JAK1 belongs to the family of non-receptor Janus tyrosine kinases (JAKs) that regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, cause the recruitment of Stat transcription factors to cytokine receptor. Subsequent phosphorylation by receptor-associated JAK causes Stat dimerization, nuclear translocation, and Stat-mediated target genes transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival, and host resistance to pathogens. Altering JAK/Stat signaling to reduce immune cells cytokine responses represents an attractive target for anti-inflammatory therapies. Although JAK1 is a ubiquitously expressed Janus kinase activated by a wide variety of cytokines, studies conducted on Jak1-knockout mice and cells indicate that JAK1 plays a non-redundant role in promoting biologic responses induced selectively by a subset of cytokine receptors.
~130 kDa observed. 133.3 kDa (human JAK1) calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
KLH-conjugated phosphopeptide corresponding to the epitope reqeion sequence containing the two phosphorylated target tyrosine residues.
Application
Detect JAK1 Tyrosine 1022 and Tyrosine 1023 phosphorylation using this rabbit polyclonal Anti-phospho-JAK1 (Tyr1022/1023), Cat. No. 07-849-I, validated for use in Dot Blot and Western Blotting
Dot Blot Analysis: A 1:1,000 dilution of this antibody detected 0.156-20 ng of the immunogen phosphopeptide, but not the corresponding non-phosphorylated peptide.
Research Category
Signaling
Biochem/physiol Actions
Predicted to react with a broad-range of mammalian species based on 100% sequence homology.
The Tyr1022/1023 designation is according to the JAK1 sequence originally published by Wilks, A.F., et al. (1991). Mol. Cell. Biol. 11(4):2057-2065 (PMID 1848670; NCBI accession: AAA36527). Numbering of the target pTyr residues varies depending on the species and source of the sequence. For example, target pTyr residues this rabbit polyclonal detects correspond to pTyr1034/pTyr1035 and pTyr1033/pTyr1034, respectively, of the human and mouse JAK1 sequence reported by UniProt (P23458 and P52332, respectively), or pTyr1033/pTyr1034 of bovine, canine, and rat JAK1 (NP_001193463 and NP_445918.1), pTyr1021/pTyr1022 of porcine JAK1 (NP_999279).
Physical form
Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:200 dilution from a representative lot detected Tyr1022/1023-phosphorylated JAK1 in 10 µg of HepG2 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Replaces: 07-849
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), canine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), porcine (based on 100% sequence homology), rat (based on 100% sequence homology) |
| technique(s) | dot blot: suitable,western blot: suitable |
| NCBI accession no. | NP_002218 |
| UniProt accession no. | P23458 |
| shipped in | ambient |
| target post-translational modification | phosphorylation (pTyr1022/pTyr1023) |
| Gene Information | human ... JAK1(3716) |
