规格
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Assay:
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Protease Assay |
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Product Line:
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EnzChek® |
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Research Category:
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Differentiation |
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Shipping Condition:
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Room Temperature |
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Substrate Properties:
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Protein-Based Substrate |
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Substrate Type:
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Protease Substrate |
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Target Enzyme:
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Proteases |
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Technique:
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Fluorescence Intensity |
储存
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Store in freezer (-5 to -30°C) and protect from light. |
The EnzChek® Protease Assay Kit, green fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with fluorescence microplate reader, is proportional to protease activity.
See our complete line of Fluorescence Microplate assays.
• Generalized mechanism allows for measurement of a variety of proteases
• Can be used to continuously measure protease kinetics
• Excitation/emission of 505/513 nm allows use of standard fluorescein (FITC) filter settings
This EnzChek® Protease Assay Kit contains a casein derivative that has been extensively labeled with the pH-insensitive, green-fluorescent BODIPY® FL dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY® FL dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The green-fluorescent BODIPY® FL dye has excitation and emission spectra similar to those of fluorescein.
