Immunogen
Epitope: cytoplasmic domain, C-terminus
Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human alpha 4 integrin subunit.
Application
Anti-Integrin α4 Antibody, cytoplasmic on C-terminus is an antibody against Integrin α4 for use in ELISA, IP, RIA, WB, IH.
ELISA/RIA: 1:1000.
Western blot: 1:1000, non-reduced conditions; blocking should be with 1-3% BSA. Recognizes a 140 kDa human alpha 4 band under non-reducing conditions.
Immunohistochemistry: 1:500
Immunoprecipitation: 5 μL of antibody is sufficient to precipitate alpha4 from 5x106 cells.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins
Biochem/physiol Actions
Integrin alpha 4. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine-labeled lymphoid cells. No cross-reactivity to alpha 1, alpha 2, alpha 3, alpha 5, alpha 6 or alpha V. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and it is thus intracellular.
Physical form
Liquid rabbit antiserum with 0.05% sodium azide.
Preparation Note
Maintain at -20ºC in undiluted aliquots for up to 12 months upon date of receipt. Avoid repeated freeze/thaw cycles.
Other Notes
Replaces: 04-1131
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human, mouse, chicken, hamster, rat, pig |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,immunohistochemistry: suitable,immunoprecipitation (IP): suitable,radioimmunoassay: suitable,western blot: suitable |
| NCBI accession no. | NM_000885.4 |
| UniProt accession no. | P13612 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | chicken ... Itga4(424121) human ... ITGA4(3676) mouse ... Itga4(16401) pig ... Itga4(100521477) rat ... Itga4(311144) |
