General description
IgG is the most abundant immunoglobulin compose of two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.
Immunogen
Epitope: Mouse IgM, IgG, IgA
Prepared from pooled antisera from goats hyperimmunized with mouse IgM, IgG and IgA paraproteins.
Application
ELISA: 1:4,000-1:8,000
Optimal working dilutions must be determined by the end user.
Goat anti-Mouse IgM+IgG+IgA (H+L) Antibody, HRP conjugate, Species Adsorbed detects level of Mouse IgM+IgG+IgA (H+L) & has been published & validated for use in ELISA.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Biochem/physiol Actions
Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.
The antibody reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.
Physical form
Affinity Chromatography
Purified by affinity chromatography on pooled mouse IgM+IgG+IgA covalently linked to agarose. Liquid in 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4.
Preparation Note
Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. Store long-term at -20°C.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | goat |
| Quality Level | 100 |
| conjugate | peroxidase conjugate |
| antibody form | affinity purified immunoglobulin |
| antibody product type | secondary antibodies |
| clone | polyclonal |
| species reactivity | mouse |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable |
| shipped in | dry ice |
| target post-translational modification | unmodified |
