General description
Sphingolipid Ceramide N-Deacylase from Pseudomonas sp. is a hydrolytic enzyme.
Application
Sphingolipid Ceramide N-Deacylase from Pseudomonas sp. has been used in enzymatic conversion and derivatization of sulfatides. It has also been used to hydrolyze the fatty acid chain of mactosyl ceramide.
Biochem/physiol Actions
Sphingolipid Ceramide N-Deacylase from Pseudomonas sp., under certain conditions, can recyclate lyso-sphingolipids. It condenses fatty acids to sphingosine to generate ceramide. The enzyme mainly acts on neutral and acidic glycosphingolipids.
Hydrolyzes the N-acyl linkage between fatty acids and sphingosines in ceramides of various sphingolipids.
Physical form
Solution in 50 mM sodium acetate, pH 6.0, with 0.1% Lubrol PX.
Other Notes
One unit will hydrolyze 1 μmol of asialo GM1 per minute at pH 6.0 at 37 °C.
| Quality Segment | 200 |
| foreign activity | exoglycosidases: α- and β-galactosidase, α- and β-N-acetylgalactosaminidase, β-N-acetylglucosaminidase, α-mannosidase, α-fucosidase, and sialidase., essentially free,protease and sphingomyelinase, essentially free |
| shipped in | dry ice |
| storage temp. | −20°C |
