General description
与人IgG结合；不与其他人Igs结合。该抗体对人IgG重链上的决定簇具有特异性。在3％ PEG 6000存在时，该抗体可在液相中形成抗原-抗体复合物。
Monoclonal Anti-Human IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulins have two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. About 70 percent of the total immunoglobulin consists of IgG.
Application
利用碱性磷酸酶偶联的小鼠抗-人IgG单克隆抗体通过Elisa对健康个体血清中针对各种金黄色葡萄球菌抗原的抗体进行检测。将抗体在PBSt中按1:30000稀释，并在37度下与样品孵育2小时。Reactin是使用对硝基苯基磷酸酯底物（Sigma）进行开发的。
液体沉淀（Lppt）。
Monoclonal Anti-Human IgG (γ-chain specific) antibody produced in mouse has been used in enzyme linked immunosorbent assay (ELISA).
Biochem/physiol Actions
Immunoglobulin G (IgG) mainly helps in immune defense. IgG participates in hypersensitivity type II and type III.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | ascites fluid |
| antibody product type | secondary antibodies |
| clone | GG-5, monoclonal |
| contains | 15 mM sodium azide |
| technique(s) | indirect ELISA: 1:20,000 |
| isotype | IgG1 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
