General description
The gene BRCA1 is mapped to human chromosome 17q21 and codes for a protein with zinc finger motif and a set of acidic residues. BRCA1 is localized to the nucleus and cytoplasm.
Immunogen
BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.
Application
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IHC, IP, ChIP. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Biochem/physiol Actions
BRCA1-6B4 recognizes full length BRCA1, a 220 kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Recent studies suggest a role for BRCA1 in DNA double strand break repair because of its association with Rad51. BRCA1 is predominantly expressed in proliferating and differentiating cells and contributes to cellular growth.
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
Physical form
Phosphate-buffered saline, no preservative added.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 6B4,monoclonal |
| form | liquid |
| mol wt | 220 kDa |
| species reactivity | mouse, human |
| concentration | 1.36 mg/mL |
| technique(s) | ChIP: suitable,immunocytochemistry: suitable,immunofluorescence: suitable,immunohistochemistry: suitable,immunoprecipitation (IP): suitable,western blot: 1:500-1:3000 |
| isotype | IgG1 |
| UniProt accession no. | P38398 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... BRCA1(672) |
