General description
The HI100 antibody reacts with the human CD45 isoform known as CD45RA, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RB, CD45RO and others.The HI100 antibody is widely used as a marker for human CD45RA expression on naïve and activated T cells, B cells, and monocytes.
Immunogen
Immunogen information for this antibody is proprietary information.
Application
Research Category
Inflammation & Immunology
This mouse monoclonal antibody Anti-CD45RA (human), PE-Cy7, clone HI100 validated for use in western blotting, IHC & Flow Cytometry for the detection of CD45RA.
Physical form
Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, <0.2% BSA, pH 7.2, and up to 0.1% sodium azide.
Size Exclusion Chromatography
Preparation Note
It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Analysis Note
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.25 µg of this antibody detected CD45RA in one million human peripheral blood lymphocytes.
Other Notes
Replaces: 04-1102
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | PE-Cy7 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | HI100, monoclonal |
| species reactivity | human |
| packaging | pkg of 5 μL (0.25 μg/test) |
| technique(s) | flow cytometry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG2bκ |
| UniProt accession no. | P08575 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PTPRC(5788) |
