General description
ATF2 (Activating Transcription Factor 2) is a member of the leucine zipper family of transcription factors. It binds to CRE domains and stimulates CRE-dependent transcription.
Mouse monoclonal anti-phospho-ATF-2 (pThr69,71) reacts specifically with ATF2 phosphorylated at both threonine 69 and 71, and does not detect the monophosphorylated and the non-phosphorylated ATF2 molecule. The epitope recognized by the antibody resides in the N-terminal region of ATF2 (aa 66-79, pT69,71). This epitope is identical in ATF2 of human, rat, mouse, chicken and frog. The antibody cross-reacts with human, rat and mouse ATF2.
Immunogen
synthetic peptide corresponding to the N-terminal amino acids 66-79 (pT69,71) of human ATF2. This sequence is identical in ATF2 from human, rat, mouse, chicken, and frog.
Application
Mouse monoclonal anti-phospho-ATF-2 (pThr69,71) antibody can be used for immunocytochemistry, indirect ELISA, microarray, and western blot.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Preparation Note
Purified from culture supernatant of hybridoma cells grown in a bioreactor.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | ATF-22P, monoclonal |
| form | buffered aqueous solution |
| mol wt | antigen 60-70 kDa (triplet) |
| species reactivity | frog, mouse, rat, chicken, human |
| concentration | 0.5-1.0 mg/mL |
| technique(s) | immunocytochemistry: suitable,indirect ELISA: suitable,microarray: suitable,western blot: suitable |
| isotype | IgG1 |
| UniProt accession no. | P15336 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pThr69/pThr71) |
| Gene Information | human ... ATF2(1386) mouse ... Atf2(11909) rat ... Atf2(81647) |
