产品介绍
Product Description
General description
Human Sirtuin 1, GenBank Accession No. NM_012238), amino acids 193-741 with N-terminal ST tag, MW = 87.2 kDa, expressed in E. coli expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.
技术参数
Specifications
| biological source | human |
| recombinant | expressed in E. coli |
| assay | ≥60% (SDS-PAGE) |
| form | aqueous solution |
| mol wt | 87.2 kDa |
| packaging | pkg of 100 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| NCBI accession no. | NM_012238 |
| UniProt accession no. | Q96EB6 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... SIRT1(23411) |
