General description
Ezrin, also known as cytovillin, p81 or 80K, is a.member of the ERM proteins family composed of Ezrin, Radixin, and Moesin. These proteins are known as actin-binding proteins that link the cell membrane to the actin cytoskeleton, particularly in microvillus-bearing epithelial cells. Ezrin is present in microvilli, microspikes, and membrane ruffles of cells.
The ERM proteins family are involved in the functional expression of membrane proteins on the cell surface, integrate Rho guanosine 5′-triphosphatase (GTPase) signaling regulating cytoskeletal organization and act as protein kinase A (PKA)-anchoring proteins. Therefore, ERM proteins seem to play an important role in the membrane transport of electrolytes by ion channels and transporters.
Immunogen
The carboxy-terminal part of recombinant human ezrin
Application
Monoclonal Anti-Ezrin recognizes an epitope located on the C-terminal region of Ezrin. Reactivity has been observed with human, mouse, hamster, monkey, bovine, canine, rat and rat-kangaroo origin. The antibody is recommended to use in various immunological techniques, including Immunoblotting (~80kDa), immunofluorescence, Immunohistochemistry and immunoprecipitation.
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Other Notes
This product is for R&D use only, not for drug, household, or other uses.
| biological source | mouse |
| conjugate | unconjugated |
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | 3C12, monoclonal |
| form | buffered aqueous solution |
| mol wt | ~80 kDa |
| species reactivity | bovine, canine, hamster, kangaroo, human, monkey, rat, mouse |
| packaging | antibody small pack of 25 μL |
| concentration | ~1 mg/mL |
| technique(s) | immunoblotting: 1-2 μg/mL using using canine MDCK cells extract,immunofluorescence: 0.5-1 μg/mL using human epidermoid carcinoma A431 cells. |
| isotype | IgG1 |
| UniProt accession no. | P15311 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Ezr(22350) |
