General description
Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
Immunogen
A synthetic phosphorylated peptide around S555 of human Phospho-ULK1-S555 (NP_003556.1).
Physical form
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | liquid |
| species reactivity | human, rat, mouse |
| concentration | 2.81 mg/mL |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | O75385 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer555) |
| Gene Information | human ... ULk1(8408) |
