General description
Neprilysin (UniProt: P08473; also known as EC:3.4.24.11, Atriopeptidase, Common acute lymphocytic leukemia antigen, CALLA, Enkephalinase, Neutral endopeptidase 24.11, NEP, Neutral endopeptidase, Skin fibroblast elastase, SFE, CD10) is encoded by the MME (also known as EPN) gene (Gene ID: 4311) in human. Neprilysin is a single-pass type II membrane glycoprotein of the peptidase M13 family that has a cytoplasmic domain (aa 2-28), a transmembrane domain (aa 29-51), and a long extracellular domain (aa 52-750). It is highly expressed on epithelial cells of lung, intestine, kidney, breast, placenta. It displays thermolysin-like specificity but is almost confined to acting on polypeptides of up to 30 amino acids. It plays an important role in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. It is also reported to degrade atrial natriuretic factor as well as brain natriuretic factor. Neprilysin is reported to protect neurons against amyloid peptide toxicity. It is considered as a cell surface marker in the diagnosis of acute lymphocytic leukemia. Glycosylation of Neprilysin at Asn-628 is shown to be essential for its surface expression and neutral endopeptidase activity. Mutations in MME gene have been linked to Charcot-Marie-Tooth disease that is characterized by progressive weakness and atrophy of peroneal muscles and distal muscles of the arms. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonal. (Ref.: Higuchi, Y., et al. (2016). Ann. Neurol. 79(4); 659-672; Wisner, A., et al. (2006). Proc. Natl. Acad. Sci. USA. 103(47); 17979-17984).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 23 amino acids from the extracellular domain of human Neprilysin/CD10.
Application
Quality Control Testing
Evaluated by Western Blotting in Mouse kidney tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Neprilysin/CD10 in Mouse kidney tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Neprilysin/CD10 in lysates from U87-MG cells and Human placenta and Rat kidney tissue.
Affinity Binding Assay: A representative lot of this antibody bound Neprilysin/CD10 with a KD of 4.6 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Neprilysin/CD10 in U-87MG cells.
Flow Cytometry Analysis: 1 µg from a representative lot detected Neprilysin/CD10 in one million Ramos cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Neprilysin/CD10 in Human kidney tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1C22 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Neprilysin/CD10. It targets an epitope within 23 amino acids from the extracellular domain.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| description | clone 1C22 ,recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 85.5 kDa,observed mol wt ~100 kDa |
| purified by | using Protein A |
| species reactivity | mouse, rat, human |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,flow cytometry: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | Extracellular domain |
| Protein ID accession no. | NP_000893.2 |
| UniProt accession no. | P08473 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... MME(4311) |
