General description
Hydrophobic ligands can serve as potentselective adsorbents. Both ω-aminoalkyl and alkyl agaroses can interact with the hydrophobic regions found in various proteins. The ω-aminoalkyl agarose product offered here consists of activated agarose with 1,6-diaminohexane covalently attached to one of its amine groups.
Application
ω-aminohexyl–agarose has been used for coupling of serotype b-specific carbohydrate antigen (SbAg) of Actinobacillus actinomycetemcomitans Y4 for isolation of anti-SbAg antibodies using protein chromatography.
ω-Aminohexyl–Agarose has been used in the conjugation of fatty acids with chain length from two carbons to 20 for fatty acyl beads pull-down experiments.
Biochem/physiol Actions
ω-aminoalkyl-agaroses are used in chromatography. In these, the protein is retained by lipophilic association between the hydrocarbon side chains on the agarose and hydrophobic pockets in the protein.
Physical form
Suspension in 0.5 M NaCl containing preservative
Other Notes
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
| biological source | plant |
| Quality Segment | 200 |
| form | saline suspension |
| extent of labeling | ≥5 μmol per mL |
| technique(s) | affinity chromatography: suitable |
| matrix | cross-linked 4% beaded agarose |
| matrix activation | cyanogen bromide |
| matrix attachment | amino of 1,6-diaminohexane |
| matrix spacer | 1 atom |
| capacity | ≥5 mg/mL binding capacity (bovine serum albumin) |
| suitability | suitable for chromatography |
| storage temp. | 2-8°C |
