General description
Antibody format: IgG F(ab′)2
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. IgA molecules in the serum are mostly dimeric. IgA is found in saliva, tracheobronchial secretions, genito urinary secretions, milk and colostrum. IgM is present on the surface of B lymphocytes in its monomeric form.
Immunogen
Mouse IgG IgA and IgM whole molecule
Biochem/physiol Actions
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. IgG2a of IgG has cytotropic properties and IgG2 molecules has the ability to activate complement.
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Goat Serum. No reaction was observed against Anti-Goat IgG F(c), Anti-Pepsin or Human Serum Proteins. This product is suitable for the detection of all Human immunoglobulin classes, isotypes and chain combinations.
Physical form
Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preparation Note
Reconstitute with 1.0 mL deionized water (or equivalent).
Legal Information
Texas Red is a registered trademark of Life Technologies
| biological source | goat |
| Quality Segment | 100 |
| conjugate | Texas Red® conjugate |
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| clone | polyclonal |
| form | lyophilized powder |
| species reactivity | mouse |
| technique(s) | direct immunofluorescence: suitable,indirect ELISA: suitable,indirect immunofluorescence: suitable |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
