Application
The enzyme from Sigma has been used as a positive control during the assay of pyruvate carboxylase activity in cell-free extracts of Corynebacterium glutamicum.
Pyruvate is critical for gluconeogenesis, lipogenesis, glyceroneogenesis, neurotransmitter biosynthesis and glucose-induced insulin, and is used to study these processes.
Biochem/physiol Actions
Pyruvate carboxylase catalyzes the carboxylation of pyruvate to oxaloacetate. Pyruvate carboxylase is a mitochondrial protein that has a biotin prosthetic group that requiries magnesium or manganese and acetyl CoA.
Physical form
Solution in 50% glycerol containing 0.05 M Tris-HCl, pH 7.4, 2 mM magnesium acetate and 1 mM EDTA.
Preparation Note
Affinity purified
Other Notes
One unit will convert 1.0 μmole of pyruvate and CO2 to oxalacetate per min at pH 7.8 at 30 °C.
| form | buffered aqueous glycerol solution |
| Quality Level | 200 |
| specific activity | 5-25 units/mg protein (BCA) |
| concentration | ≥0.5 mg/mL |
| foreign activity | lactic dehydrogenase ≤0.5% |
| storage temp. | −20°C |
