General description
Monoclonal Anti-TIN2 (mouse IgG1 isotype) is derived from the hybridoma TIN-30 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human TIN-2, conjugated to keyhole limpet hemocyanin (KLH). The six shelterin subunits are: TRF1, TRF2, and POT1 which directly recognize the TTAGGG repeats, and TIN2, Rap1 and TPP1. TIN2 (TRF-1 interacting nuclear factor 2) is recruited to the telomere through the TRF homology (TRFH) domain of TRF1 and tethers TPP1/POT1 to TRF1 and TRF2.
Application
Monoclonal Anti-TIN2 antibody produced in mouse has been used in immunoblotting.
Biochem/physiol Actions
TIN2 (TRF-1 interacting nuclear factor 2) also connects TRF1 to TRF2 and this link contributes to the stabilization of TRF2 on telomeres. In addition, TIN2 has also been found to tether the telomeres to the nuclear matrix. Interestingly, this protein has also been found to be mutated in Dyskeratosis Congenita, a multi-system disorder which in its classical form is characterized by abnormalities of the skin, nails, and mucous membranes.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | TIN30, monoclonal |
| form | buffered aqueous solution |
| mol wt | antigen ~40 kDa |
| species reactivity | human |
| packaging | antibody small pack of 25 μL |
| concentration | ~1.0 mg/mL |
| technique(s) | western blot: 0.5-1.0 μg/mL using HeLa cell extract |
| isotype | IgG1 |
| UniProt accession no. | Q9BSI4 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... TINF2(26277) |
