General description
Protein tyrosine phosphatase nonreceptor, type 1 (PTPN1) is the founding member of the protein tyrosine phosphatase (PTP) family. PTPN1 catalyze the hydrolysis of the phosphate monoesters specifically from tyrosine residues. PTPN1 is known to be involved in signaling pathways that regulate a variety of cellular processes including cell growth and oncogenic transformation. PTPN1 also has been shown to act as a negative regulator of insulin signaling. PTPN1 has also been reported to dephosphorylate epidermal growth factor receptor kinase, which implicated the role of this phosphatase in cell growth control and cell response to interferon stimulation.
Physical form
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
| recombinant | expressed in baculovirus infected Sf9 cells |
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| specific activity | 2125-2875 nmol/min·mg |
| mol wt | ~71 kDa |
| NCBI accession no. | NM_002827 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... PTPN1(5770) |
