General description
Haptoglobin, also known as HP, is a serum α2-glycoprotein that exists as a tetramer, composed of two smaller identical alpha (α) chains and two larger identical beta (β) chains. The α-chains are linked to each other by a disulfide bond and each β-chain is similarly linked to an α-chain.
Monoclonal Anti-Haptoglobin specifically recognizes the three types of human haptoglobin (types 1-1, 2-1, and 2-2) The antibody recognizes both purified and haptoglobin in human serum, and shows no crossreactivity with human IgG, transferrin, C-reactive protein, α-1-acid glycoprotein, albumin, hemoglobin, or bovine, goat and sheep serum haptoglobin.
Immunogen
purified haptoglobin from pooled human plasma
Application
The antibody is recommended to use in various immunological techniques, including ELISA, dot blot, immunoprecipitation, and immunoblot . The antibody reacts against native and denatured (non-reduced) human haptoglobin but does not recognize the haptoglobin under reducing conditions.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative.
Other Notes
This product is for R&D use only, not for drug, household, or other uses.
| biological source | mouse |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | HG-36, monoclonal |
| form | buffered aqueous solution |
| species reactivity | human |
| packaging | antibody small pack of 25 μL |
| concentration | ~1.0 mg/mL |
| technique(s) | ELISA: 0.2-0.4 μg/mL using 10 μg/ml human haptoglobin for coating,dot blot: suitable,immunoblotting: suitable,immunoprecipitation (IP): suitable |
| isotype | IgG1 |
| UniProt accession no. | P00738 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... HP(3240) |
