General description
Programmed Death-1 (PD-1, CD279) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses. Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or downregulate T cell activation (CTLA-4 and PD-1). CD279 is expressed on activated T-cells, B-cells, myeloid cells and on a subset of thymocytes. In vitro, ligation of CD279 inhibits TCR-mediated T-cell proliferation and production of IL-1, IL-4, IL-10 and IFN-γ. In addition, CD279 ligation also inhibits BCR mediated signaling. CD279 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases.
Physical form
Lyophilized from 0.2 μm-filtered solution in PBS.
Preparation Note
Reconstitute at 100 μg/mL in sterile PBS.
Other Notes
The extracellular domain of human CD279 [PD-1] (aa 25-167) is fused to the N-terminus of the Fc region of human IgG1.
| biological source | human |
| recombinant | expressed in CHO cells |
| assay | ≥98% (SDS-PAGE) |
| form | lyophilized |
| mol wt | monomer 46 kDa by calculation |
| packaging | pkg of 100 μg |
| impurities | <0.06 EU/μg endotoxin, tested |
| UniProt accession no. | Q15116 |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | human ... PDCD1(5133) |
