Application
Useful for kinase assay and Western blot.
Biochem/physiol Actions
Recombinant full-length human LIMK2 was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. LIMK2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain (1). LIM domains are highly conserved cysteine-rich structures that can mediate protein-protein interaction. LIMK2 is phosphorylated and activated by ROCK, a downstream effector of Rho, and then LIMK2 in turn phosphorylates cofilin, inhibiting its actin-depolymerizing activity which contributes to Rho-induced reorganization of the actin cytoskeleton (2). LIMK2 is highly expressed in liver, colon, stomach, spleen, brain, kidney, and placenta.
Preparation Note
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
| recombinant | expressed in baculovirus infected Sf9 cells |
| assay | 70-95% (SDS-PAGE) |
| form | frozen liquid |
| specific activity | 2.5-3.5 nmol/min·mg (radiometric) |
| mol wt | ~96 kDa |
| concentration | 0.05 μg/μL |
| accession no. | CR456513 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... LIMK2(3985) |
