General description
Plexin-A3 is a cell surface receptor for semaphorin that controls signaling for neuronal axon regulation. Plexin-A3 is thought to play a role in Sema3F and Sema3A signaling, has been described as essential for dorsal turning of the corticospinal tract axons and may be an important contributor to development of hippocampal axonal projections. Plexin-A3 has been found expressed in newly-differentiated sympathetic neurons, but not their neural crest precursors, and together with Plexin-A4, functions cooperatively to regulate the migration of sympathetic neurons and then differentially to guide the sympathetic axons.
~220 kDa observed. The calculated molecular weight of this protein is 208 kDa; however, a higher band may be observed due to glycosolation.
Immunogen
GST-tagged recombinant protein corresponding to human Plexin-A3.
Application
Anti-Plexin-A3 Antibody, clone 18A9.2 is a Mouse Monoclonal Antibody for detection of Plexin-A3 also known as PLEXIN-A3, Semaphorin receptor SEX or Plexin-4 & has been validated in WB & IHC.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Western Blot Analysis: 1 µg/mL from a previous lot detected Plexin-A3 on 10 µg of HT-29 and NIH/3T3 cell lysates.
Immunohistochemistry Analysis: 1:400 dilution from a previous lot detected Plexin-A3 in colorectal carcinoma tissue.
Physical form
Format: Purified
Protein G
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
PC-12 cell lysate
Evaluated by Western Blot in PC-12 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Plexin-A3 on 10 µg of PC-12 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 18A9.2, monoclonal |
| species reactivity | rat, mouse, human |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_059984 |
| UniProt accession no. | P51805 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PLXNA3(55558) |
