General description
The Transcreener® UDP2 FP Assay is a universal biochemical HTS assay for enzymes that produce UDP, including glycosyltransferase, galactosyltransferase, glucuronyltransferase, N-acetylglucosamyltransferase, N-acetlygalactosyltransferase, xylosyltransferase, and glycogen, cellulose, lactose and hyaluronan synthases. Enzyme activity is signaled by a decrease in fluorescence polarization as the bound tracer is displaced from the Transcreener® UDP2 Antibody. The assay is a simple single step mix-and-read format enabling the use of unmodified native substrate concentrations of 1 – 1000 μM. The assay provides excellent signal at low substrate conversion, with a Z′ =0.7 and =100 millipolarization shift (mP) under normal reaction conditions.
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Physical form
Kit with buffered aqueous solutions
Preparation Note
3018-1K = 1,000 assay, 384-well
3018-10K = 10,000 assay, 384-well
Legal Information
Transcreener is a registered trademark of BellBrook Labs
| shipped in | dry ice |
| storage temp. | −20°C |
