General description
The 2H7 antibody reacts with human CD20, expressed by pre-B cells, resting and activated B cells, follicular dendritic cells and a small subset of T cells, but not on plasma cells. CD20 is a 33-37 kDa transmembrane protein which is differentially phosphorylated in resting and active B cells. The CD20 molecule plays a role in regulating B-cell activation.The 2H7 antibody is reported to be cross-reactive with several non-human species including Baboon, Chimpanzee, Cynomolgus and Rhesus.
Immunogen
Immunogen information for this antibody is proprietary information.
Application
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This mouse monoclonal Anti-CD20 Antibody (human), violetFluor® 450, clone 2H7, Cat. No. MABF1639 is validated for use in Flow Cytometry, for the detection of CD20.
Physical form
Purified Mouse monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Analysis Note
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.5 μg from a representative lot detected CD20 in one million human peripheral blood lymphocytes.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
violetFluor is a registered trademark of Tonbo Biotechnologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | violetFluor® 450 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2H7, monoclonal |
| species reactivity | human |
| technique(s) | flow cytometry: suitable |
| UniProt accession no. | P11836 |
| target post-translational modification | unmodified |
| Gene Information | human ... MS4A1(931) |
