Anti-Notch1 antibody, Mouse monoclonal，200UL，N6786-200UL，Sigma

General description

Recognizes human and mouse Notch1 (~300 kDa and 120 kDa) once Notch1 is cleaved. The antibody does not recognize Notch 2, 3, or 4. It has high affinity for the activated intracellular form of Notch.
The Notch protein family is a group of highly conserved proteins important in the determination of cell fate and maintenance of progenitors in many developmental systems.In mammals, four Notch genes were identified (Notch1-4) in a wide variety of cells and play a crucial role in differentiation and development. Notch proteins contain three main domains: an extracellular domain containing tandem epidermal growth factor repeats, and three lin-12/Notch repeats, a transmembrane domain, and an intracellular domainthat contains six cdc1-/SW16/ankyrin repeats, nuclear localization signals, and a C-terminal OPA/PEST region.

Immunogen

recombinant protein containing the cdc10-NCR region of mouse Notch1.

Application

Anti-Notch1 antibody, mouse monoclonal has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry
• immunohistochemistry

Biochem/physiol Actions

NOTCH is a ubiquitous and essential component of the extracellular matrix (ECM) and plays a vital role in tissue repair mechanism. Fibronectin functions both as a regulator of cellular processes and as an important scaffolding protein to maintain and direct tissue organization and ECM composition.Fibronectin is suggested to enhance cell adhesion, spreading and affect the routes of cell migration both in vivo and in culture. It has been shown that upon malignant transformation many cells lose most of their surface bound fibronectin.
Recognizes human and mouse Notch1 (~300 kDa and 120 kDa) once Notch1 is cleaved. The antibody does not recognize Notch 2, 3, or 4. It has high affinity for the activated intracellular form of Notch.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.