General description
APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth, is involved in monocyte/macrophage-mediated immunological processes and functions as an important survival factor for plasmablasts and bone marrow plasma cells. APRIL is a high activity construct in which two trimeric APRIL ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively stimulates proliferation B cell. A basic amino acid sequence (QKQKKQ) close to the N-terminus of APRIL is required for binding to negatively charged sulfated glycosaminoglycan side chains of proteoglycans. Proteoglycans mediate binding of APRIL to tumor cells as well as primary lymphoid cells.
Physical form
Lyophilized from PBS.
Preparation Note
Reconstitute with 100 μL deionized water.
Other Notes
The extracellular domain of mouse APRIL (aa 88-232) is fused at the N-terminus to mouse ACRP30headless (aa 18-110) and a FLAG®-tag.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | mouse |
| recombinant | expressed in HEK 293 cells |
| tag | FLAG® tagged |
| assay | >95% (SDS-PAGE) |
| form | lyophilized |
| mol wt | ~34 kDa by SDS-PAGE |
| packaging | pkg of 10 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| impurities | <0.02 EU/μg endotoxin, tested |
| color | white |
| UniProt accession no. | Q9D777 |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | mouse ... Tnfsf13(69583) |
