General description
This product is prepared by labeling highly cross-adsorbed donkey anti-chicken IgY (H+L) with CF350. CF350 is a blue fluorescent dye spectrally similar to the traditional blue fluorescent dye, AMCA. However, compared to AMCA, CF350 is more water-soluble, more photostable and at least 50% more fluorescent on proteins. CF350 is at least as bright as Alexa fluor 350 and thus can be a direct replacement for the latter for all applications.
Immunogen
chicken IgY (H+L)
Biochem/physiol Actions
Binds all chicken IgYs (minimal cross-reaction with bovine, goat, guinea pig, syrian hamster, horse, human, mouse, rabbit, rat and sheep proteins)
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
Physical form
Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 0.2% BSA.
Preparation Note
Protect from light. The antibody solution should be gently mixed before use.
Legal Information
This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
CF is a trademark of Biotium, Inc.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | donkey |
| Quality Segment | 100 |
| conjugate | CF™ 350 conjugate |
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| species reactivity | chicken |
| concentration | ~2 mg/mL |
| technique(s) | flow cytometry: 1-10 μg/mL,immunocytochemistry: suitable,immunohistochemistry: suitable,indirect ELISA: suitable,indirect immunofluorescence: 1-10 μg/mL,western blot: suitable |
| fluorescence | λex 347 nm; λem 448 nm |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
