General description
Plakoglobin is a desmosomal protein that regulates developmental signalling in vertebrates. Tyrosine phosphorylation of plakoglobin has been linked to adenocarcinomas . Plakoglobin may function as a tumor suppressor in ovarian and breast cancers .
Immunogen
recombinant chicken plakoglobin.
Application
Monoclonal anti-plakoglobin (catenin γ) antibody is suitable for use in immunocytochemistry (using MDCK cells), immunoprecipitation, and in immunoblotting . The antibody may also be used for immunohistochemistry (frozen sections), microarray, western blot (1:500, using cultured MDBK cells) and indirect immunofluorescence (1:1000, using cultured MDBK cells).
Biochem/physiol Actions
The antibody recognizes the plakoglobin (catenin-γ) molecule (85 kDa and possibly a slightly lower band) by immunoblotting. It does not cross-react with β-catenin. The antibody reacts with plakoglobin in bovines, humans and dogs, but does not react with β catenin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| clone | 15F11, monoclonal |
| form | buffered aqueous solution |
| mol wt | antigen 85 kDa |
| contains | 15 mM sodium azide |
| species reactivity | bovine, canine, human |
| technique(s) | immunocytochemistry: suitable using cultured cells,immunohistochemistry (frozen sections): suitable,immunoprecipitation (IP): suitable,indirect immunofluorescence: 1:1,000 using cultured MDBK cells,microarray: suitable,western blot: 1:2,000 using cultured MDBK cells |
| isotype | IgG1 |
| UniProt accession no. | P14923 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... JUP(3728) |
