Biochem/physiol Actions
Interleukin-7 is a lymphoid cell growth factor which affects pre-B, pro-B and early T cells. It supports the growth of early B cells and is mitogenic for thymocytes and co-mitogenic with PHA and Con A. IL-7 stimulates the proliferation of CD4/CD8 cells. Human and mouse IL-7 have 60% amino acid sequence identity.
Interleukin-7 is a lymphoid cell growth factor which affects pre-B, pro-B and early T cells. It was first isolated by Namen in 1988. IL-7 has a number of known activities. It supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic for thymocytes and co-mitogenic with PHA and Con A. IL-7 also stimulates the proliferation of CD4/CD8 cells. The proliferative response of thymocytes to IL-7 is not affected by antibodies to the T cell growth factors such as IL-2, IL-4, and IL-6. Mature T cells respond to IL-7 and Con A, but not to IL-7 alone. Antibodies against IL-2 affect its activity, suggesting that it functions through IL-2 production. Human natural IL-7 is 25 kDa glycoprotein that has 6 cysteine residues which are important for biological activity. Human and mouse IL-7 have 60% amino acid sequence homology.
Lymphoid cell growth factor which affects pre-B, pro-B and early T cells; supports the growth of early B cells and is mitogenic for thymocytes and co-mitogenic with PHA and Con A; stimulates the proliferation of CD4/CD8 cells.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin.
| biological source | mouse |
| Quality Level | 100 |
| recombinant | expressed in E. coli |
| assay | ≥97% (SDS-PAGE) |
| form | lyophilized powder |
| packaging | pkg of 5 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| technique(s) | cell culture | mammalian: suitable |
| impurities | endotoxin, tested |
| UniProt accession no. | P10168 |
| storage temp. | −20°C |
| Gene Information | mouse ... Il7(16196) |
