General description
Anti-Histone H3 (Acetyl-Lys18) Antibody detects endogenous levels of total Histone H3 (Acetyl-Lys18) protein.
Histone H3 is located on chromosome 6p22. Mammals contain three main classes of histone H3 variants: the replicative histones (H3.1 and H3.2), the replacement histone (H3.3) and the centromeric histone (Cenp-A).
Immunogen
The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys18.
Immunogen Range: 1-50
Application
Anti-Histone H3 (Acetyl-Lys18) antibody has been used in enzyme-linked immunosorbent assay (ELISA).
Biochem/physiol Actions
Histone proteins are basic building blocks of chromatin. Mutations in Histone H3 results in adult cerebellar high-grade gliomas. It controls protein-protein interactions to induce binding of trans-acting factors that “drive” chromatin condensation.
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
Physical form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| mol wt | antigen 15 kDa |
| species reactivity | mouse, rat, human |
| concentration | ~1 mg/mL |
| technique(s) | ELISA: 1:5000,immunofluorescence: 1:100-1:500,immunohistochemistry: 1:50-1:100,western blot: 1:500-1:1000 |
| NCBI accession no. | P68431 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | acetylation (Lys18) |
| Gene Information | human ... HIST1H3A(8350) |
