General description
Leucyl-cystinyl aminopeptidase (UniProt: Q9UIQ6; also known as EC:3.4.11.3, Cystinyl aminopeptidase, Insulin-regulated membrane aminopeptidase, Insulin-responsive aminopeptidase, IRAP, Oxytocinase, OTase, Placental leucine aminopeptidase, P-LAP) is encoded by the LNPEP (also known as OTASE) gene (Gene ID: 4012) in human. LENPEP/IRAP is a zinc-dependent aminopeptidase that cleaves vasopressin, oxytocin, dynorphin A and other peptide hormones and is involved in the inactivation of neuronal peptides. It is a single-pass type II membrane protein with a cytoplasmic domain (aa 1-110), a transmembrane domain (aa 111-131), and a large extracellular domain (aa 132-1025). It is highly expressed in placenta, heart, kidney, and small intestine. Lower levels are detected in neuronal cells, skeletal muscle, spleen, liver, testes, and colon. In the brain tissue only a membrane-bound form is reported. It resides in intracellular vesicles together with GLUT4. and can then translocate to the cell surface in response to insulin and/or oxytocin. Cleavage of membrane-bound LENPEP/IRAP between residues 154-155 generates a pregnancy serum form that plays a role in maintaining homeostasis during pregnancy. Three isoforms of LENPEP/IRAP have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Matsumoto, H., et al. (2001). Eur. J. Biochem. 268(11); 3259-3266; Tsujimoto, M., et al. (1992). Arch. Biochem. Biophys. 292(2); 388-392).
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 16 amino acids from the cytoplasmic domain of human Leucyl-cystinyl aminopeptidase (LNPEP).
Application
Quality Control Testing
Evaluated by Western Blotting in Ramos cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LNPEP/IRAP in Ramos cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LNPEP/IRAP in lysates from HeLa cells, C6 cells, and Mouse heart tissue..
Affinity Binding Assay:: A representative lot of this antibody bound LNPEP/IRAP peptide with a KD of 3.9 x 10-7 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected LNPEP/IRAP in HeLa cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected LNPEP/IRAP in Human cerebral cortex tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1G23 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Leucyl-cystinyl aminopeptidase (LNPEP). It targets an epitope within 16 amino acids from the cytoplasmic domain.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| description | Clone, 1G23 |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 117.35 kDa,observed mol wt ~170 kDa |
| purified by | using Protein A |
| species reactivity | mouse, rat, human |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | Cytoplasmic domain |
| Protein ID accession no. | NP_005566 |
| UniProt accession no. | Q9UIQ6 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... LNPEP(4012) |
