General description
Integrin beta1 (UniProt: P05556; also known as Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12) gene (Gene ID: 3688) in human. Integrins are heterodimeric cell surface receptors composed of a variable alpha subunit and a conserved beta subunit. The exact combination of various alpha and beta subunits dictates the binding specificity of integrins to different ECM components. Integrins are not constitutively active. Activation of an integrin from low ligand-binding affinity state to high ligand-binding affinity state requires conformational change that can originate from either from their cytoplasmic or extracellular interactions. Integrin beta-1 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off to produce the mature form. The mature form contains an extracellular domain (aa 21-728), a transmembrane domain (aa 729-751), and a cytoplasmic domain (aa 752-798). Integrin beta-1 heterodimerizes with either alpha-1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 or alpha-V to recognize different ligands. For example, alpha-1/beta-1 can recognize the proline hydroxylated sequence in collagen and alpha-2/beta-1 can serve as receptor for fibronectin. It is shown that beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Five different isoforms of integrin beta-1 have been described that are produced by alternative splicing. Isoform 1 is shown to be widely expressed while other isoforms are generally co-expressed with a more restricted distribution. Also, isoform 2 is reported to Interfere with isoform 1 resulting in a dominant negative effect on cell adhesion and migration. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Immunogen
KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal, cytoplasmic domain of human Integrin beta1, isoform 1.
Application
Anti-Integrin beta1, clone 1A27 ZooMAb, Cat. No. ZRB1230, is a recombinant Rabbit monoclonal antibody that specifically targets Integrin beta1 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Integrin 1 in COS7, HUVEC, and C6 cell lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin 1 in HeLa, A431, HUVEC, and NIH 3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Integrin 1 in human uterus and human prostate cancer tissue sections.
Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.
Biochem/physiol Actions
Clone 1A27 is a ZooMAb rabbit recombinant monoclonal antibody that detects Integrin beta1. It targets an epitope within 23 amino acids from the C-terminal, cytoplasmic domain.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit (recombinant) |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1A27, recombinant monoclonal |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 88.41 kDa,observed mol wt ~120 kDa |
| species reactivity | mouse, rat, human, monkey |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable,western blot: suitable |
| isotype | IgG |
| UniProt accession no. | P05556 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... ITGB1(3688) mouse ... Itgb1(16412) rat ... Itgb1(24511) rhesus monkey ... Itgb1(574312) |
