General description
Protein G binds to antibodies reversibly and thus, it is considered in antibody purification. It is a 20 kDa protein.
Protein G is an immunoglobulin (IgG) specific bacterial cell wall protein isolated from Streptococci (group G). Native proteins G consist of two IgG-binding domains and albumin and cell surface binding sites. Biotin is a water soluble vitamin that acts as a coenzyme for carboxylase enzyme reactions. Biotin deficiency results in deregulation of metabolic pathways and various physiological impairments. Biotin deficiency is known to cause birth anomalies indicating its important role in development and differentiation of embryos.
Application
Protein G-Biotin from Streptococcus sp. has been used in electrochemical biosensing, for the attachment of antibodies with different specificities.
Protein G-Biotin from Streptococcus sp. has been used during incubation for synergistic immunocapture of antigen which was performed using streptavidin-coated magnetic beads.
Biochem/physiol Actions
Binds IgG only from most mammalian species except cat; binds chicken IgG.
Packaging
Package size based on protein content
Preparation Note
Prepared using biotinamidocaproate N-hydroxysuccinimide which incorporates an aminocaproyl spacer.
Other Notes
Truncated - lacks albumin, Fab and membrane binding sites.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| recombinant | expressed in unspecified host |
| Quality Level | 200 |
| conjugate | biotin conjugate |
| form | lyophilized powder |
| composition | Protein, ~90% biuret |
| extent of labeling | 2-4 mol d-biotin per mol protein |
| technique(s) | direct ELISA: 1:70,000 using human IgG |
| storage temp. | 2-8°C |
