General description
The antibody neutralizes receptor-ligand interaction. At 0.5-2 μg/mL the antibody will block 50% of the binding of 5 ng/mL recombinant human HGF to 0.1 μg immobilized recombinant human HGF R/c-MET Fc chimera. >90% of the binding will be blocked by 10 μg/mL of the antibody.
Immunogen
purified recombinant human hepatocyte growth factor receptor extracellular domain expressed in mouse NSO cells.
Application
Anti-Hepatocyte Growth Factor Receptor (c-Met) antibody produced in goat was used to block c-Met in HeLa cells.
Biochem/physiol Actions
Hepatocyte Growth Factor (HGF) is a multifunctional, heparin-binding factor that stimulates the proliferation of liver cells. The cellular effects of HGF are mediated by its receptor, c-Met, that leads to significant activation of ERK, Src/FAK, STAT3 and PI3K signaling. Various functions of the HGF/c-Met signaling include proliferation, cell survival, motility and adhesion and tissue regeneration. Apart from liver, HGF also mediates regeneration of kidney, lung and gastro-intestines.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | goat |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | lyophilized powder |
| species reactivity | human |
| technique(s) | flow cytometry: suitable,immunohistochemistry: 5-15 μg/mL,western blot: 0.1-0.2 μg/mL |
| impurities | endotoxin, tested |
| UniProt accession no. | P08581 |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... MET(4233) mouse ... Met(17295) |
