General description
Goat Anti-Mouse IgM(µ chain) Biotin Alpaca Heavy chain only Antibody is designed to specifically detect mouse IgM. It is a monoclonal, recombinant, goat IgG Fc fused single domain antibody to µ chain of mouse IgM coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Goat Anti-Mouse IgM(µ chain) Biotin Alpaca Heavy chain only Antibody reacts with the µ chain of mouse IgM selectively but not with mouse, rabbit, human, cynomolgus, rat, goat IgG.
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. IgM accounts for 5-10% of the immunoglobulin pool and is the predominant antibody in the primary immune response. Unlike IgG, IgM does not contain a hinge region but does contain an additional constant domain. The monomeric form IgM has a molecular weight of 180 KD. It is classically represented as a pentamer of the basic four chain structure held together by a J chain but can also exist in a hexameric form without the J chain and as a monomer on the surface of B-cells.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Immunogen
Recombinant Alpaca single variable domain secondary nano antibody
Biochem/physiol Actions
Fc region of mouse IgM (µ chain specific)
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc
Packaging
100 μg in Glass bottle
Physical form
Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose
Preparation Note
Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL
After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C
Other Notes
No cross-reactivity with mouse, rabbit, human, cynomolgus, rat, goat IgG
| biological source | alpaca |
| recombinant | expressed in HEK 293 cells (Human cell line) |
| conjugate | biotin |
| antibody form | affinity purified immunoglobulin |
| antibody product type | secondary antibodies |
| clone | recombinant monoclonal |
| form | lyophilized solid |
| mol wt | 100 kDa |
| species reactivity | Alpaca, mouse |
| technique(s) | ELISA: 1:5000-1:20000,western blot: 1:5000-1:20000 |
| size | 0.1 mL |
| color | colorless |
| isotype | VHH |
| shipped in | wet ice |
| storage temp. | 2-8°C |
