General description
PH Domain And Leucine Rich Repeat Protein Phosphatase 2 is encoded by the PHLPP2 gene. It is known to catalyze the dephosphorylation of pSer473 of AKT1 and pSer660 of PRKCbeta-II, as well as pSer657 of PRKCalpha.
~150 kDa observed. Predicted to cross-react to all isoforms. Isoform 2 (~107kDa) can possibly be detected at longer exposures/higher concentrations or in certain tissues.
Immunogen
Epitope: Within PP2C-like Domain
KLH-conjugated linear peptide corresponding within the PP2C-like domain of human PHLPP2.
Application
Anti-PHLPP2 Antibody is an antibody against PHLPP2 for use in Western Blotting and Immunocytochemistry.
Immunocytochemistry Analysis: 2.0 ug/mL from a representative lot detected PHLPP2 in HUVEC cells.
Research Category
Signaling
Research Sub Category
Signaling Neuroscience
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HUVEC cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected PHLPP2 in 10 µg of HUVEC cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | bovine (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_055835 |
| UniProt accession no. | Q6ZVD8 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PHLPP2(23035) |
