General description
BAFF, a member of the TNF superfamily of ligands, is expressed in T cells, macrophages, monocytes and dendritic cells. BAFF is involved in stimulation of B and T cell function, and is an important survival and maturation factor for peripheral B cells. BAFF signals through three different TNF receptors TACI, BCMA and BAFF-R. The human BAFF gene codes for a 285 amino acid type II transmembrane protein containing a 46 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 218 amino acid extracellular domain. Recombinant human soluble BAFF is a 152 amino acid polypeptide (17.0 kDa), which contains the TNF-like portion of the extracellular domain of BAFF.
Biochem/physiol Actions
BAFF, a member of the TNF superfamily of ligands, is expressed in T cells, macrophages, monocytes and dendritic cells. Recombinant human soluble BAFF is a 152 amino acid polypeptide (17.0 kDa), which contains the TNF-like portion of the extracellular domain of BAFF.
Physical form
Lyophilized from 20 mM Tris, pH 8.0 + 150mM NaCl.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
| biological source | human |
| recombinant | expressed in E. coli |
| assay | ≥98% (HPLC),≥98% (SDS-PAGE) |
| form | lyophilized |
| mol wt | 17.0 kDa |
| packaging | pkg of 20 μg |
| impurities | endotoxin, tested |
| UniProt accession no. | Q9Y275 |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | human ... TNFSF13B(10673) |
