General description
Lysine-specific demethylase 6A (UniProt: O15550; also known as EC:1.14.11.68, Histone demethylase UTX, Ubiquitously-transcribed TPR protein on the X chromosome, Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein, [histone H3]-trimethyl-L-lysine(27) demethylase 6A, KDM6A, UTX) is encoded by the KDM6A (also known as UTX) gene (Gene ID: 7403) in human. UTX is a histone demethylase that specifically demethylates lysine 27 of histone H3 and plays a central role in histone code. It is shown to demethylate trimethylated and dimethylated, but not monomethylated H3 lysine 27. It mediates muscle regeneration through its demethylase activity, while it regulates mesoderm differentiation of embryonic stem cells and mammary gland development independent of its demethylase activity. It also plays a role in regulating brown adipocyte thermogenic program. Its levels are shown to be up-regulated during brown adipocyte differentiation and by cold exposure in both brown and white adipose tissue in mice, indicating its potential role in thermogenesis. Mutations in KDM6A gene are a causative factor in Kabuki syndrome that is characterized by congenital mental retardation, postnatal dwarfism, large prominent earlobes, a cleft or high-arched palate, scoliosis, and other abnormalities. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zha, L., et al. (2015). J. Biol. Chem. 290(41); 25151-25163; Lederer, D., et al. (2012). Am. J. Hum. Genet. 90(1); 119-124).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 19 amino acids from the internal region of human Lysine-specific demethylase 6A (KDMA6/UTX).
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected UTX in HeLa cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected UTX in HaCat and COS-1 cell lysate.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected UTX in HeLa cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected UTX in human bone marrow tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected UTX in HeLa cell lysate.
Biochem/physiol Actions
Clone 4G9 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Lysine-specific demethylase 6A (KDMA6/UTX). It targets an epitope within 19 amino acids from the internal region.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 4G9, recombinant monoclonal |
| description | 4G9 Clone |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 154.18 kDa,observed mol wt ~160 kDa |
| purified by | using Protein A |
| species reactivity | human, monkey |
| species reactivity (predicted by homology) | mouse, rat |
| packaging | antibody small pack of 25 μL |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | Internal |
| Protein ID accession no. | NP_066963 |
| UniProt accession no. | O15550 |
| greener alternative category | , Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| Gene Information | human ... KDM6A(7403) |
