General description
SMYD2 (also known as SET and MYND domain-containing protein 2) is a member of the SMYD family of protein methyltransferases. SMYD2 is also known as lysine methyltransferase protein 3C (KMT3C). SMYD2 functions to repress transcription by interacting with the Sin3A histone deacetylase complex and performs methylation of Lys36 in histone H3. The exogenous expression of SMYD2 in mouse fibroblasts decreases cell growth. SMYD2 also function as a putative oncogene by methylating p53 and repressing its tumor suppressive function.
Physical form
Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Preparation Note
After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.
| recombinant | expressed in baculovirus infected Sf9 cells |
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~70 kDa |
| NCBI accession no. | BC049367 |
| shipped in | dry ice |
| storage temp. | −70°C |
| Gene Information | human ... SMYD2(56950) |
